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With the completion of sequencing projects and the advancement of a- lyrical tools for protein identification, the proteomics-the study of the expressed part of the genome-has become a major region of the burgeoning field of functional genomics.

High-resolution 2-D gels can reveal virtually all p- teens present in a cell or tissue at any given time, including posttranslationally modified proteins.

Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols cover all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectrometry-based protein identification methodologies.

This book is intended as a benchtop manual and guides both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry-especially in the fields of molecular biology, biochemistry, microbiology, and cell biology-should find this book eminently useful.

2-D Proteome Analysis Protocols take the researcher through the c- plate process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.