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Prestigious researchers from ten different countries present readily reproducible experimental protocols to study the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these cutting-edge techniques offer novel approaches, such as the use of genome databases and siRNA, to analyzing how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. An earlier first volume, Reviews and Model Systems, comprehensively reviews the complexities of checkpoint controls and the model systems needed to study them.
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