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Biochemistry terms were first introduced by Carl Neuberg in 1903. The field of biochemistry encompasses the chemistry of life and living processes. It is not an exaggeration to state that the scope of biochemistry is as extensive as life itself. Every facet of life—birth, growth, reproduction, aging, and death—involves intricate biochemical processes. Indeed, every movement in life is characterized by numerous biochemical reactions. Biochemistry stands out as the most rapidly advancing and innovative subject in the field of medicine. The discipline of biochemistry serves as a guiding light, enabling us to explore the intricate complexities of biology and unravel the chemical mysteries of life. Biochemical research consistently reveals that all living organisms are closely interconnected at the molecular level. Therefore, biochemistry serves as a unifying subject within the diverse living kingdom. Advancements in biochemistry exert a profound impact on human welfare and significantly contribute to improving the quality of life. This impact is evident in various areas, such as the application of biochemistry in laboratory diagnostics for diseases and the development of products such as insulin, interferon, growth hormone, etc. through genetic engineering. Additionally, the potential use of gene therapy in the near future is another promising avenue made possible by advances in biochemistry. Organization of the Book This Textbook comprises of 9 chapters, that is organized around the following major themes: Conformation of biomolecules, structure and their relationship to biological activity. Synthesis and degradation of major metabolites. Production and storage of energy. Biocatalysts and their application. Intercellular communication by hormones. Molecular events in gene expression and regulation